Analysis Note

ControlNIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)

Application

Research Sub CategoryPI3K, Akt, & mTOR Signaling

Research CategorySignaling

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1, Magnetic Bead Conjugate validated for use in IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Immunogen

GST-fusion protein corresponding to residues 1-149 of human Akt1

Physical form

20% slurry; 1X PBS, 0.05% sodium azide.
Sufficient for 10 IPs.

Protein G Chromatography

Format: Purified

Quality

Evaluated by immunoprecipitation on 250 µg of NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes).

Specificity

Recognizes Akt1/PKBα.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 60 kDa observed